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https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/febd4c20-4d12-401d-b011-dfc6d002bc7b/Other+examples+button.jpg https://www.nextgenseqanalysis.com/en/past-projects/project-six-sz8wl-6setl monthly 0.5 2023-02-05 https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/a4b6fc2a-1bcd-4075-a938-50fa2cd53eef/micro+heatmap.jpg NextGenSeek custom NGS analysis: Past projects - Micro RNAs and short non-coding RNA Heatmap to show the pathways affected by the most significantly miRNAs https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/b1d3f43c-00b2-4e55-bd03-21a6979e7713/Volcano+Micro.jpg NextGenSeek custom NGS analysis: Past projects - Micro RNAs and short non-coding RNA Volcano plot showing the most significantly different miRNAs https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/54cbf2d7-62e8-4034-a292-2b404b472a02/micro+disease+ontology.jpg https://www.nextgenseqanalysis.com/en/past-projects/project-four-yjynj-9wdc7 monthly 0.5 2023-12-21 https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/81e652df-12c6-4318-9e55-d937bd9e2b2f/Screenshot+2024-11-24+at+15.23.24.png https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/5798d423-0b52-4e52-8bb3-da6d771722d7/elife-27596-fig4.jpg https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/f8fa4aab-695d-47e4-92fe-7bbaa4361f0d/pgen.1009873.g005.jpg https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/3bb342b4-b721-4409-8a1d-c913e855ca54/survival+plot.png https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/da959160-9b3f-43b7-ad19-4f5d7b06edfb/Cytoscape+plot.png https://www.nextgenseqanalysis.com/en/past-projects/project-three-8zgh7-bwktc monthly 0.5 2023-02-05 https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/3ee534bd-0df0-4c09-a5aa-178fdb346451/off+target.jpg NextGenSeek custom NGS analysis: Past projects - Gene editing: on-target and off-target sequencing On and off-target effects of gene editing (Silva-Pinheiro et al. 2022) https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/1670971525930-WC4WQJH2ZEQ5HI2D825I/off%2Btarget%2B1.jpg NextGenSeek custom NGS analysis: Past projects - Gene editing: on-target and off-target sequencing https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/580c96df-5182-473c-948f-90e968e74a3b/off+target+3.jpg NextGenSeek custom NGS analysis: Past projects - Gene editing: on-target and off-target sequencing https://www.nextgenseqanalysis.com/en/past-projects/project-three-8zgh7-bwktc-fh8as monthly 0.5 2023-03-26 https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/431083ee-cd56-4f4e-8581-516ba697d3d2/Heatmap+RNASeq+everolimus.jpg NextGenSeek custom NGS analysis: Past projects - RNA-Seq and pathway analysis Heatmaps are often a good way to show changes between samples (Lambros et al. 2023, IJMS) https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/84cebc18-7ebf-4d3b-b9a7-2426f3c14d71/Venn+diagram.jpg NextGenSeek custom NGS analysis: Past projects - RNA-Seq and pathway analysis Venn diagram showing differentially expressed genes and similarities between groups https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/68b668e1-acfc-47c7-b459-53e28f553f0a/DE+pathways.jpg NextGenSeek custom NGS analysis: Past projects - RNA-Seq and pathway analysis Pathway differences https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/ab1dd6d4-006c-41f3-a1f9-6cd4bb56e84e/Screenshot+2022-07-12+at+16.14.07.png NextGenSeek custom NGS analysis: Past projects - RNA-Seq and pathway analysis Screenshot of a gene in a genome browser, clearly showing the different introns and exons. https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/9f90011a-193f-42ce-98ea-0a48f5fc301e/Screenshot+2023-02-25+at+17.32.46.png NextGenSeek custom NGS analysis: Past projects - RNA-Seq and pathway analysis Volcano Plot showing differentially expressed genes with colour indication of the relevant genes for this study https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/1514497a-3e7d-43b4-ba5a-cc46edffd495/TEFM.jpg NextGenSeek custom NGS analysis: Past projects - RNA-Seq and pathway analysis Example of a coverage analysis on mitochondrial RNA (Van Haute et al. 2023, Nature Communications) https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/1660674547505-BLLR20UIWHESKZKKO3CC/Cancer+pathway+example+2.jpg https://www.nextgenseqanalysis.com/en/past-projects/project-three-8zgh7-bwktc-3flrn monthly 0.5 2023-02-05 https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/1663608683721-S69RR5LKWESWBQEJUUVX/Splicing.jpg NextGenSeek custom NGS analysis: Past projects - RNA alternative splicing https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/6b7444c9-7c97-41a0-bcb0-cc4a8e75054a/SMA+splicing+summary.jpg NextGenSeek custom NGS analysis: Past projects - RNA alternative splicing Bar chart showing differences in splicing https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/9f337d14-81be-4b8e-9c94-d045dcdf3104/SMA+switching+genes+summary.jpg NextGenSeek custom NGS analysis: Past projects - RNA alternative splicing graph showing alternative splicing events https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/e17796c1-2564-4977-ad4a-4daf9fcc3707/CNversusCP.difftest_alt_3prime.3329.jpg NextGenSeek custom NGS analysis: Past projects - RNA alternative splicing Example of alternative splicing analysis https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/2d5224d1-5fc5-4b92-9896-a9c48177fecb/sashimi.jpg NextGenSeek custom NGS analysis: Past projects - RNA alternative splicing Sashimi plot https://www.nextgenseqanalysis.com/en/past-projects/metagenomics monthly 0.5 2023-02-05 https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/c8683a86-2aa3-432c-b924-ddbd6fe0d446/metagenomics+fig2.jpg NextGenSeek custom NGS analysis: Past projects - Metagenomics https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/78b8452e-f5a8-4c93-ad4b-2cdc22047d8f/metagenomics+fig3.jpg NextGenSeek custom NGS analysis: Past projects - Metagenomics https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/5b280d2f-263d-48fb-a0cb-1f9a2c329b67/metagenomics+fig5.jpg https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/7f4ef761-2027-4820-b093-0f030887935f/metagenomics+fig4.jpg https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/629fe195-2398-4151-8cd5-a1278022090f/metagenomics+fig1.jpg https://www.nextgenseqanalysis.com/en/past-projects/scrna-seq monthly 0.5 2023-02-05 https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/1b3d9eab-7b6e-4ef0-9b28-87e8f3265895/sc21.jpg NextGenSeek custom NGS analysis: Past projects - Single cell RNA-Seq (scRNA-seq) https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/c68bcaf3-d95c-4c85-8249-c20ec8e6534f/sc14.jpg NextGenSeek custom NGS analysis: Past projects - Single cell RNA-Seq (scRNA-seq) https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/1f626935-6a7f-4996-9288-af66dc59232c/pseudotime.jpg NextGenSeek custom NGS analysis: Past projects - Single cell RNA-Seq (scRNA-seq) Compare gene patterns between cell types https://images.squarespace-cdn.com/content/v1/62fa5a44fe91e2716e935f5e/b3e12d49-286b-445c-bf0b-dea6125ac3f0/Go+dotplot.jpg NextGenSeek custom NGS analysis: Past projects - Single cell RNA-Seq (scRNA-seq) Dot plot image showing the most significantly activated and suppressed pathways 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