RNA alternative splicing is a crucial biological process that generates various variants of a single gene from a common primary transcript. This mechanism enables the production of multiple protein products by selectively including or excluding different exons from the final mature RNA. The regulation of alternative splicing involves a complex interplay of trans-acting factors and cis-acting elements, including splice sites, enhancers, and silencers. These elements collectively determine the inclusion or exclusion of specific exons in the resulting mRNA.

Widespread in eukaryotic cells, alternative splicing is estimated to impact over 90% of human genes. This process is instrumental in allowing cells to diversify their protein repertoire, generating a multitude of proteins from a relatively limited set of genes. Beyond contributing to proteomic diversity, alternative splicing plays a pivotal role in gene expression regulation and has implications for various biological processes, including the development of diseases such as cancer.

If you seek in-depth analysis of alternative splicing events within your next-generation sequencing data, we invite you to contact us. Our expertise in genomic analysis can provide valuable insights into the intricate landscape of alternative splicing, contributing to a comprehensive understanding of gene expression dynamics.