Epigenomics and epitranscriptomics

NGS allows for the high-throughput profiling of DNA methylation patterns, histone modifications, and chromatin accessibility, providing a comprehensive view of the epigenetic landscape. Bioinformatics tools then meticulously analyze this data, offering insights into the regulatory elements governing gene expression, cellular differentiation, and the development of various diseases.

The study of tRNA modifications holds significant relevance in understanding the molecular basis of diseases. tRNA modifications influence the fidelity and efficiency of protein translation, and disruptions in these modifications are increasingly implicated in various pathological conditions. NGS technologies, coupled with bioinformatics analyses, enable researchers to comprehensively map tRNA modifications, facilitating the identification of alterations associated with diseases such as cancer, neurodegenerative disorders, and metabolic diseases.

A selection of our publications

Van Haute L, et al. (2021) Detection of 5-formylcytosine in mitochondrial transcriptome. Methods Mol Biol 2192:59-68 doi: 10.1007/978-1-0716-0834-0_5

Van Haute L, et al. (2019) METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis. Nucleic Acids Res 47(19):10267-10281 doi: 10.1093/nar/gkz735

Van Haute L, et al. (2019) NSUN2 introduces 5-methylcytosines in mammalian mitochondrial tRNAs. Nucleic Acids Res 47(16):8720-8733 doi: 10.1093/nar/gkz559

Van Haute L, et al. (2017) Dealing with an unconventional genetic code in mitochondria: the biogenesis and pathogenic defects of the 5-formylcytosine modification in mitochondrial tRNAMet. Biomolecules 7(1):24 doi:10.3390/biom7010024

Van Haute L, et al. (2016) Deficient methylation and formylation of mt-tRNAMet wobble cytosine in a patient carrying mutations in NSUN3. Nat Commun 7:12039 doi: 10.1038/ncomms12039

Van Haute L, et al. (2015) Mitochondrial transcript maturation and its disorders. J Inherit Metab Dis 38:655-80 doi:10.1007/s10545-015-9859-z

And read our blog about the mysteries of RNA methylation